Figure 4. Tankyrase knockdown impairs the translocation of IRAP and GLUT4 but not GLUT1.
Day 6 adipocytes were electroporated with T1A siRNA or a point-mutation control (Pmt). On day 8, (A) whole-cell extracts (30 μg protein/lane) and (B) the PM fractions (15 μg protein/lane) were immunoblotted and quantified as described in Figure 2(C). The bar graph to the left shows the means±S.E.M. of two basal samples and of four insulin-stimulated samples. *P<0.05. This experiment was repeated twice with similar results.