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X represents the position of the artificial abasic site (tetrahydrofuran residue). (A) linear 60/17-mer substrate; (B) linear 60/17-mer substrate containing the abasic site; (C) circular 100/17-mer substrate; (D) circular 100/17-mer substrate containing an abasic site for running start experiments; (E) circular 100/18-mer substrate containing an abasic site for standing start experiments.
