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. 2007 Feb 12;402(Pt 2):321–329. doi: 10.1042/BJ20060898

Figure 2. Replication of linear DNA substrates by gp43 wt and gp43D219A exo− DNA polymerases.

Figure 2

(A) PhosphorImage of the reaction products for undamaged substrate, A, or substrate that contains an AP lesion. Reactions were performed as described in the Materials and methods section with 30 nM substrates A or B for 30 min at 37 °C at the indicated concentrations of polymerases. In the case of lanes 5–8 and 9–12, concentrations of the enzymes were 0.01 nM, 0.1 nM, 1 nM and 10 nM respectively. The position of the 17-mer (primer) and of the 60-mer (full-length product) are indicated on the left-hand side of the gel, while the location of the abasic site is indicated on the right-hand side. (B) Quantification of the data shown in (A) for the damaged substrate.