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. 2007 Feb 12;402(Pt 2):321–329. doi: 10.1042/BJ20060898

Figure 3. Replication of circular DNA substrates by gp43 wt and gp43D219A exo− DNA polymerases in a running start reaction.

Figure 3

(A) PhosphorImage of the reaction products. Reactions were performed as described in the Materials and methods section with 15 nM of running start substrates C (undamaged) or D (with an AP lesion) for 30 min at 37 °C at the indicated concentrations of polymerases. In the case of lanes 6–10 and 11–15, concentrations of the enzymes were 0.001 nM, 0.01 nM, 0.1 nM, 1 nM and 10 nM respectively. The position of the 17-mer (primer) and of the 100-mer (full-length product) are indicated on the left-hand side of the gel, while the location of the abasic site is indicated on the right-hand side. (B) Quantification of the data shown in (A) for the damaged substrate.