Figure 4. Insertion of a nucleotide in front of the lesion of an AP-containing circular DNA substrate in a running start reaction.

(A) PhosphorImage of the reaction products. Running start reactions were performed as described in the Materials and methods section with 15 nM substrate D at the indicated times and concentrations of polymerases. The position of the 17-mer (primer) is indicated on the left-hand side of the gel while the location of the abasic site is indicated on the right-hand side. (B) Quantification of the data shown in (A).