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. 2007 Feb 12;402(Pt 2):321–329. doi: 10.1042/BJ20060898

Figure 6. Replication of the AP-containing circular DNA substrate by gp43wt and gp43D219A exo− DNA polymerases, in combination with other replicative proteins, in both running and standing start reactions.

Figure 6

(A) Reactions were performed as described in the Materials and methods section with 15 nM running start or standing start substrate D, 0.05 nM of either gp43 wt or gp43D219A exo− polymerases, 100 nM of gp45, 200 nM of gp44/62 and 150 nM of gp32. The incubation time was 30 min at 37 °C. The position of the 17-mer primer, the AP site and the 100-mer full-length product for the running start reaction are indicated on the left-hand side of the gel, while the location of the 18-mer primer and of the abasic site for the standing start reaction are indicated on the right-hand side. (B) Quantification of the extension from the AP site by the D219A exo− polymerase, either alone or in combination with the replicative proteins. Data are the average of four independent experiments±S.E.M.