Reactivities of syphilis patient sera and control sera from uninfected individuals and individuals with other spirochetal diseases to the recombinant T. pallidum proteins. The y axis shows the mean ELISA absorbance values at 600 nm for reactivities of the sera to the recombinant Tp0453, Tp92, and Gpd proteins. On the x axis the sera are grouped as follows: U, uninfected laboratory personnel sera (n = 15); L, leptospirosis patient sera (n = 9); LD, Lyme disease patient sera (n = 8); RF, relapsing fever patient sera (n = 8); and S, syphilis patient sera (n = 43). The overall mean absorbance of each group is represented by a horizontal line.