TABLE 1.
ORF-specific primers used to amplify fragments for recombinant expressiona
| ORF | Sense primer | Antisense primer | Amplicon detail
|
|
|---|---|---|---|---|
| Size (bp) | Portion of ORF | |||
| Tp0155 | 5′-GGATCCAACCATTGACACCTGCC | 5′-GAATTCTGCAGCTGAATTATAGAAC | 428 | 5′ end |
| Tp0257 (Gpd) | 5′-GGATCCTGCGGGGAACATATTGTGTG | 5′-GAATTCATACGGGGCGGGTTTGCC | 1,067 | Full |
| Tp0326 (Tp92) | 5′-CAGGCAAACGACAATTGG | 5′-CAAATTATTTACCGTGAACG | 2,448 | Full |
| Tp0453 | 5′-GGATCCCGTGGAAGGCATCAGTAG | 5′-GAATTCCGAACTTCCCTTTTTGGAG | 758 | Full |
| Tp0483 | 5′-GGATCCAGGAACTCGTCCACGTATC | 5′-GAATTCGTTATGAAAGCGATAGCCG | 1,058 | Full |
| Tp0751 | 5′-GGATCCGGGACACCGCCGCACAC | 5′-GAATTCCTTGCGGTGTGTGTGCGC | 360 | 5′ end |
a
Restriction sites incorporated into the primers are indicated by underlining.