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. 2007 Feb 12;402(Pt 2):387–395. doi: 10.1042/BJ20061096

Figure 3. Oct-2 transactivates the resistin promoter.

Figure 3

Wild-type (A, B and C) or Octamer-mutated (D) reporter plasmid pResistin(−996/+61)-Luc was transfected into RAW264.7 cells. Various amounts of expression vectors for Oct-1 (pCG-Oct-1), Oct-2 (pCG-Oct-2) or the empty vector (pCG) were co-transfected as indicated. At 20 h after transfection, cells were either left untreated (■) or treated with 10 ng/ml LPS (□) for 4 h. Oct-1 protein, detected by Western blot analysis, in cells transfected with various amounts of pCG-Oct-1 plasmid is shown in the bottom of (A). The relative luciferase activity is the activity relative to that of cells transfected with pCG alone (A, B and D) or pCG and pCG-Oct-2 only (C) in the absence of LPS. Results are means±S.D. for at least three independent experiments. *P<0.05 compared with cells transfected only with pCG in (A), (B) and (D). *P<0.05 compared with cells transfected with pCG-Oct-2 and pCG, but no pCG-Oct-1, in (C).