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. 2007 Feb 12;402(Pt 2):387–395. doi: 10.1042/BJ20061096

Figure 7. Knockdown of Oct-2 inhibits the expression of resistin in response to LPS treatment.

Figure 7

(A) The pLL3.7-Oct-2 plasmid was transfected into RAW264.7 cells using the SuperFect reagent. At 44 h after transfection, the cells were left untreated or were treated with 100 ng/ml LPS for 4 h, then Oct-2, Oct-1, resistin, iNOS and GAPDH mRNA were detected by RT–PCR as described in the Experimental section. (B) RAW264.7 cells were co-transfected with pResistin(−996/+61)-Luc and pLL3.7-Oct-2 plasmids for 24 h, then were either left untreated or were treated with LPS for 4 h. Fold activation (+LPS/−LPS) of luciferase activity by LPS treatment is shown. Results are means±S.D. for three independent experiments. pLL, pLL3.7 plasmid; Sc, pLL3.7-scrambled plasmid. *P<0.05 compared with the pLL3.7 or scramble control.