Oligonucleotides used for real-time PCR |
|
|
Resistin |
Forward |
CTGATGTCGGGGAAGTGAGC |
|
Reverse |
GTGCAGGTGCCTGTAGAGAC |
GAPDH |
Forward |
ACCCACTCGTCCACCTTTGA |
|
Reverse |
CATACCAGGAAATGAGCTT |
Oligonucleotides used for RT–PCR |
|
|
Oct-1 |
Forward |
CCTGCAACCAGCACAGTTTA |
|
Reverse |
CTACGATTCAAGCCCTCAGC |
Oct-2 |
Forward |
AATGGACCCGACATTAACCA |
|
Reverse |
AAATGGTCGTTTGGCTGAAG |
iNOS |
Forward |
AGGAACATCTGGCCAGGCTG |
|
Reverse |
ACTTGGGATGCTCCATGGTC |
GAPDH |
Forward |
AAAGGATCCACTGGCGTCTTCACCACC |
|
Reverse |
GAATTCGTCATGGATGACCTTGGCCAG |
Oligonucleotides used for mutagenesis |
|
|
C/EBP-mt |
Forward |
ACGCGTCTTTTCATTTGTaCgcTATAAACTTCCTCTGCTA |
|
Reverse |
TAGCAGAGGAAGTTTATAgcGtACAAATGAAAAGACGCGT |
Ets-mt |
Forward |
TGTCCAATTTATAAACacagTCTGCTAATCTCAATTTTGT |
|
Reverse |
AAAATTGAGATTAGCAGActgtGTTTATAAATTGGACAAATG |
Oct-mt |
Forward |
CTGACGTTAACACCCAGGgccTGCATAGCCACCTCCAGTT |
|
Reverse |
AACTGGAGGTGGCTATGCAggcCCTGGGTGTTAACGTCAG |