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. 2007 Feb 12;402(Pt 2):387–395. doi: 10.1042/BJ20061096

Table 1. Sequences of the oligonucleotides used.

Cis-elements are underlined. The lower-case letters indicate the mutated sequences.

Oligonucleotide Sequence (5′→3′)
Oligonucleotides used for real-time PCR
 Resistin Forward CTGATGTCGGGGAAGTGAGC
Reverse GTGCAGGTGCCTGTAGAGAC
 GAPDH Forward ACCCACTCGTCCACCTTTGA
Reverse CATACCAGGAAATGAGCTT
Oligonucleotides used for RT–PCR
 Oct-1 Forward CCTGCAACCAGCACAGTTTA
Reverse CTACGATTCAAGCCCTCAGC
 Oct-2 Forward AATGGACCCGACATTAACCA
Reverse AAATGGTCGTTTGGCTGAAG
 iNOS Forward AGGAACATCTGGCCAGGCTG
Reverse ACTTGGGATGCTCCATGGTC
 GAPDH Forward AAAGGATCCACTGGCGTCTTCACCACC
Reverse GAATTCGTCATGGATGACCTTGGCCAG
Oligonucleotides used for mutagenesis
 C/EBP-mt Forward ACGCGTCTTTTCATTTGTaCgcTATAAACTTCCTCTGCTA
Reverse TAGCAGAGGAAGTTTATAgcGtACAAATGAAAAGACGCGT
 Ets-mt Forward TGTCCAATTTATAAACacagTCTGCTAATCTCAATTTTGT
Reverse AAAATTGAGATTAGCAGActgtGTTTATAAATTGGACAAATG
 Oct-mt Forward CTGACGTTAACACCCAGGgccTGCATAGCCACCTCCAGTT
Reverse AACTGGAGGTGGCTATGCAggcCCTGGGTGTTAACGTCAG