Table 2. Data sampling requirements: the effect of sampling rate on parameter values.
Progress curves for aryl-acylamidase, collected over 25 min and at ten different temperatures, were used to generate the Equilibrium Model parameters as described in the Experimental section. Experimental data points were then successively removed to give the effect of reduced frequency of data points to determine the effect of various sampling rates on the final parameter values. Parameter values are means±S.D.
| Sampling interval (s)… | 1 | 5 | 20 | 60 | 150 | |
|---|---|---|---|---|---|---|
| Parameter | Data points per progress curve… | 1500 | 300 | 75 | 25 | 10 |
| ΔG‡cat (kJ·mol−1) | 74.4±0.01 | 74.4±0.02 | 74.4±0.03 | 74.4±0.06 | 74.4±0.09 | |
| ΔG‡inact (kJ·mol−1) | 94.5±0.04 | 94.5±0.09 | 94.5±0.18 | 94.5±0.31 | 94.5±0.48 | |
| ΔHeq (kJ·mol−1) | 138.5±0.6 | 138.5±1.4 | 138.5±2.8 | 138.7±4.8 | 138.8±7.4 | |
| Teq (K) | 310.0±0.1 | 310.0±0.1 | 310.0±0.2 | 310.0±0.3 | 310.0±0.5 |