Table 3. Final parameter values for an enzyme employing a discontinuous assay.

Acid phosphatase was assayed discontinuously over a period of 30 min with a sampling rate of 60 s and at 5 °C intervals from 20 to 80 °C (13 temperature points). The results of fitting data for the same enzyme over the same temperature range and using the same intervals, but using a continuous assay (effective sampling rate of 1 s) have been included for comparison [2]. The progress curves generated for both methods were fitted to the Equilibrium Model and the parameters generated as described in the Experimental section. Parameter values are means±S.D.

Parameter	Discontinuous assay	Continuous assay
ΔG‡cat (kJ·mol−1)	79.0±0.02	79.1±0.01
ΔG‡inact (kJ·mol−1)	96.1±0.23	94.5±0.04
ΔHeq (kJ·mol−1)	146.0±2.2	142.5±0.5
Teq (K)	333.6±0.5	336.9±0.1