Table 4. Data sampling requirements: the effect of temperature range on parameter values.
A full set of experimental data for acid phosphatase was used to generate the Equilibrium Model parameters as described in the Experimental section. Temperature points above the Topt (Figure 2) were sequentially truncated from the complete data set to determine the influence of data points above the Topt on the final parameter values. Temperature points were also sequentially truncated from the lowest temperature point to the highest from the complete data set (12 temperature points) to determine how many points, in total, below the Topt are required for the accurate determination of Teq and the other thermodynamic parameters. In this case, each data set included all temperature points above the Topt. Parameter values are means±S.D.
| Truncated from highest temperature point | Truncated from lowest temperature point | ||||||
|---|---|---|---|---|---|---|---|
| Parameter | Minus three temperature points (nine points) | Minus two temperature points (ten points) | Minus one temperature point (11 points) | Full data set (12 points) | Minus two temperature points (ten points) | Minus four temperature points (eight points) | Minus six temperature points (six points) |
| ΔG‡cat (kJ·mol−1) | 78.8±0.01 | 79.1±0.01 | 79.1±0.01 | 79.1±0.01 | 79.1±0.01 | 79.0±0.01 | 79.3±0.02 |
| ΔG‡inact (kJ·mol−1) | 94.3±0.03 | 94.6±0.05 | 94.6±0.05 | 94.5±0.04 | 94.5±0.05 | 94.5±0.05 | 94.2±0.06 |
| ΔHeq (kJ·mol−1) | 108.5±0.5 | 148.8±0.7 | 146.5±0.5 | 142.5±0.5 | 142.8±0.6 | 142.1±0.7 | 149.8±0.8 |
| Teq (K) | 337.3±0.1 | 336.8±0.1 | 336.8±0.1 | 336.9±0.1 | 337.0±0.1 | 336.9±0.1 | 338.3±0.2 |