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. 2006 Aug 11;66(2):151–157. doi: 10.1136/ard.2006.054056

Table 2 Flow cytometry staining protocol.

Phycoerythin Fluorescein isothiocyanate Peridinin–chlorophyll protein
CD14 CD3 CD45
CCR2 CCR5− biotin+ avidin CD14
CCR2 CXCR3 CD4
CD14 Avidin CD14
Nonsense IgG CD4 CD3
Nonsense IgG Nonsense IgG CD14

CCR, CC chemokine receptor; CXCR, CXC chemokine receptor; IgG, immunoglobulin G.

Staining with CCR5‐biotinylated avidin–fluorescein isothiocyanate did not yield signals different from the background.