Table 2 Flow cytometry staining protocol.
Phycoerythin | Fluorescein isothiocyanate | Peridinin–chlorophyll protein |
---|---|---|
CD14 | CD3 | CD45 |
CCR2 | CCR5− biotin+ avidin | CD14 |
CCR2 | CXCR3 | CD4 |
CD14 | Avidin | CD14 |
Nonsense IgG | CD4 | CD3 |
Nonsense IgG | Nonsense IgG | CD14 |
CCR, CC chemokine receptor; CXCR, CXC chemokine receptor; IgG, immunoglobulin G.
Staining with CCR5‐biotinylated avidin–fluorescein isothiocyanate did not yield signals different from the background.