TABLE 1.
G and P type distribution of the rotavirus strains detected in Bari in 2001-2002a
| No. of sample | VP7 G type | VP4 P type and lineage | VP6 genogroup |
|---|---|---|---|
| 13 | G9 | P[8] Malawian-like | II |
| 1 | G9b | P[8]b | II |
| 2 | G9b | ND | ND |
| 5 | G1 | P[8] Japanese-like | II |
| 7 | G1 | P[8] American-like | II |
| 1 | G1 + G9b | P[8]b | ND |
| 1 | ND | P[8]b | ND |
a
Thirty samples positive by a quick diagnostic test were subjected to molecular analysis. ND, not determined. Of the 30 samples, 29 were typed for VP7 6 type, 28 were typed for VP4 p type and lineage, and 26 were typed for VP6 genogroup.
b
The VP7 and VP4 specificities were determined by a typing assay based on a heminested PCR with type-specific primers, since poor amplification of the first-round PCR did not allow sequence analysis.