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. 2003 Aug;41(8):3548–3558. doi: 10.1128/JCM.41.8.3548-3558.2003

FIG. 5.

FIG. 5.

CfoI-generated T-RFLP profiles formed from 16S rRNA gene PCR products (700IR labeled) amplified from DNA extracted directly from a sputum sample separated on a LI-COR IR2 automated sequencer. Lane 1 and 12, microSTEP 15a ladder (sizes in bases). Lanes 2 to 6 were loaded with equal quantities of CfoI-digested T-RFLP PCR products amplified from five separate DNA extractions from a single sputum sample; lanes 7 to 11 were loaded with equal quantities of CfoI-digested T-RFLP PCR products amplified in five separate reactions with a template from a single DNA extraction.