TABLE 1.
Summary of genotypic and phenotypic characterization results obtained in this study for cpb2-positive C. perfringens isolates
| Source and strain | Type | cpb2 PCR resulta | cpb2 RFLP pattern (kb) with HpaI | CPB2-specific Western blotting resultsb | cpb2 location determined by PFGE | Reference |
|---|---|---|---|---|---|---|
| cpb2-positive strain CWC245 | C | + | ∼5 | + | Plasmid | 10 |
| Healthy pigs | ||||||
| JGS1537 | A | − | − | − | NDc | 9 |
| JGS1540 | A | − | − | ND | ND | 9 |
| JGS1569 | A | − | − | ND | ND | 9 |
| JGS1580 | A | − | − | ND | ND | 9 |
| D9703881 | A | − | − | ND | ND | 9 |
| 106527 | C | − | − | − | − | 17 |
| Diarrheic pigs | ||||||
| 106526 | A | + | ∼5 | + | Plasmid | 17 |
| JGS1528 | A | + | ∼5 | + | ND | 9 |
| JGS1538 | A | + | ∼5 | + | ND | 9 |
| JGS1541 | A | + | ∼5 | + | Plasmid | 9 |
| JGS1548 | A | + | ∼6 | + | ND | 9 |
| JGS1552 | A | + | ∼5 | + | Plasmid | 9 |
| JGS1561 | A | + | ∼5 | + | Plasmid | 9 |
| JGS1570 | A | + | ∼5 | + | ND | 9 |
| JGS1580 | A | − | − | ND | ND | 9 |
| JGS1807 | A | + | ∼5 | + | Plasmid | 9 |
| JGS1817 | A | + | ∼5 | + | Plasmid | 9 |
| JGS1818 | A | ± | >20 | + | Plasmid | 9 |
| 120-98 | A | + | ∼5 | + | Plasmid | 9 |
| 170-98 | A | + | ∼5 | + | Plasmid | 9 |
| 855-97#3 | A | + | ∼5 | + | ND | 9 |
| 855-97#4 | A | + | ∼5 | + | Plasmid | 9 |
| 2011 | A | + | ∼8 | + | Plasmid | 9 |
| 2142 | A | + | ∼5 | + | ND | 9 |
| 9461-97 | A | + | ∼5 | + | Plasmid | 9 |
| 9777colon | A | + | ∼5 | + | Plasmid | 9 |
| 10618 J | A | + | ∼5 | + | Plasmid | 9 |
| 10728 | C | + | ∼5 | + | ND | 9 |
| 97-4029 | C | + | ∼6 | + | ND | 9 |
| JGS1071 | C | + | ∼5 | + | Plasmid | 9 |
| JGS1090 | C | + | ∼5 | + | Plasmid | 9 |
| JGS1495 | C | + | ∼5 | + | ND | 9 |
| JGS1504 | C | + | ∼5 | + | ND | 9 |
| JGS1508 | C | + | ∼5 | + | ND | 9 |
| 106640 | C | + | ∼5 | + | Plasmid | 17 |
a
+, presence of both 1,373-bp and 318-bp products; −, absence of both 1,373-bp and 318-bp PCR products; ±, presence of 318-bp product but absence of 1,373-bp product.
b
+, presence of ∼28-kDa immunoreactive band; −, absence of ∼28-kDa immunoreactive band.
c
ND, not determined.