TABLE 1.
Phenotypic and genotypic identification results for 49 optochin-resistant alpha-hemolytic pneumococcus-like Streptococcus spp.
| Group and study isolate no. | Original isolate no. | AccuProbe result | Capsule formation | PCR result
|
ARDRA patterna | Bile solubility | ||
|---|---|---|---|---|---|---|---|---|
| psaA | lytA | ply | ||||||
| Group I | ||||||||
| STR119 | KTL 004 | + | + | + | + | + | p | + |
| STR120 | KTL 005 | + | + | + | + | + | ND | + |
| STR125 | KTL 013 | + | + | + | + | + | p | + |
| STR127 | KTL 017 | + | + | + | + | + | ND | + |
| STR141 | KTL 043 | + | + | + | + | + | p | + |
| STR144 | KTL 051 | + | + | + | + | + | ND | + |
| STR147 | KTL 056 | + | + | + | + | + | ND | + |
| STR149 | KTL 063 | + | + | + | + | + | p | + |
| STR155a | KTL 076 | + | + | + | + | + | ND | + |
| STR163 | KTL 091 | + | + | + | + | + | ND | + |
| STR164 | KTL 093 | + | + | + | + | + | ND | + |
| Group IIa | ||||||||
| STR157 | KTL 079 | + | − | − | wb | + | p | − |
| STR148b | KTL 062b | + | − | − | w | + | p | − |
| STR150 | KTL 065 | + | − | − | w | + | p | − |
| STR162 | KTL 089 | + | − | − | − | + | p | − |
| STR165 | KTL 096 | + | − | − | w | + | p | + |
| STR142b | KTL 046b | + | − | − | − | + | p | + |
| STR146b | KTL 055b | + | wc | − | − | − | p | − |
| Group IIb | ||||||||
| STR122 | KTL 007 | − | − | − | − | + | m | − |
| STR133 | KTL 028 | − | − | − | − | + | ND | − |
| STR126b | KTL 016b | − | − | − | w | + | m | − |
| STR129 | KTL 020 | − | − | − | w | + | m | − |
| STR130 | KTL 021 | − | − | − | − | + | ND | + |
| STR137 | KTL 035 | − | − | − | w | + | m | + |
| STR152 | KTL 069 | − | − | − | w | + | m | + |
| STR153 | KTL 072 | − | − | − | − | + | m | + |
| STR166b | KTL 099b | − | − | − | − | + | m | + |
| STR131 | KTL 022 | − | − | − | w | − | m | + |
| STR151 | KTL 068 | − | − | − | − | − | ND | + |
| Group IIc | ||||||||
| STR118 | KTL 003 | − | − | − | − | − | ND | − |
| STR121 | KTL 006 | − | − | − | − | − | ND | − |
| STR136 | KTL 034 | − | − | − | − | − | ND | − |
| STR138 | KTL 038 | − | − | − | − | − | ND | − |
| STR145 | KTL 054 | − | − | − | − | − | ND | − |
| STR154 | KTL 073 | − | − | − | − | − | ND | − |
| STR123 | KTL 008 | − | − | − | − | − | ND | − |
| STR128 | KTL 019 | − | − | − | − | − | m | − |
| STR132 | KTL 023 | − | − | − | − | − | ND | − |
| STR134 | KTL 029 | − | − | − | − | − | ND | − |
| STR139 | KTL 039 | − | − | − | − | − | m | − |
| STR158 | KTL 081 | − | − | − | − | − | ND | − |
| STR159 | KTL 083 | − | − | − | − | − | ND | − |
| STR156 | KTL 077 | − | − | − | − | − | m | − |
| STR117 | KTL 002 | − | − | − | − | − | ND | − |
| STR140 | KTL 041 | − | − | − | − | − | ND | − |
| STR160 | KTL 085 | − | − | − | − | − | ND | − |
| STR161 | KTL 087 | − | − | − | − | − | ND | − |
| STR135 | KTL 030 | − | w | − | − | − | m | − |
| STR143 | KTL 050 | − | w | − | − | − | m | − |
a
ARDRA, PCR-based amplification of the 16S rRNA-gene followed by RsaI digestion, agarose electrophoresis, and ethidium bromide staining. m, S. mitis-specific pattern, p, S. pneumoniae-specific pattern, ND, not determined.
b
Weak band after agarose gel electrophoresis and ethidium bromide staining.
c
Weak capsule reaction, with the strain considered unencapsulated.