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Fluorescence images of a single MCF-7 cell (LH-RH receptor-positive), taken 4 min (a), 20 min (b), 40 min (c), and 50 min (d) after treatment with AN-152:C625 (0.6 μM). The staining of the cell occurred within 5 min, and the entry into the nucleus occurred after 20 min, with an increase of the fluorescence intensity over time.
