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. 2003 Jul 8;4:5. doi: 10.1186/1471-2091-4-5

Figure 3.

Figure 3

Complex formation and specific inhibitory activity of PAI-1s Panel A: The PAI-1s were incubated for 30 min at 37°C in the absence or in the presence of a two molar excess of either single-chain tPA or uPA. They were then subjected to SDS-PAGE and immunobloting using anti-PAI-1 monoclonal antibodies. Lanes 1, 4, 7, 10 are PAI-1s only, lanes 2, 5, 8, 11 are PAI-1s with tPA, and lanes 3, 6, 9, 12 are PAI-1s with uPA. The filled arrow indicates the complexes between PAI-1s and tPA, and the regular arrow indicates the complex between PAI-1s and uPA. Panel B: The specific inhibitory activity of D355A, D355H, D355K, D355E, D355Q, and D355N towards uPA was measured immediately after their purification and after their activation with 6 M Gdn-HCl as described under Methods. The data represent the mean ± SE of three separate determinations.