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. 2003 Jul 8;4:5. doi: 10.1186/1471-2091-4-5

Figure 6.

Figure 6

Inhibition of plasmin by wtPAI-1, E351A and E351R Plasmin (50 ul, 100 nM) was incubated with equal amount of wtPAI-1, E351A, or E351R alone in term of inhibitory activity toward uPA (0, 0.32, 1.6 and 8 unit in 50 ul, respectively, upper panel), or in presence of two molar excess of vitronectin(lower panel). Residual plasmin activity was calculated according to the absorbance at 405 nm after 60 minutes incubation with plasmin substrate (final concentration, 0.5 mM). The activity of plasmin without PAI-1s was taken as 100%.