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. 1999 Sep 28;96(20):11122–11127. doi: 10.1073/pnas.96.20.11122

Figure 3.

Figure 3

Receptor multiplex assays. (Left) Different receptors on common particles. Cy5.5+ and Cy5+ (a), Cy5.5+, (b) or Cy5+ (c) fluorescence events detected for indicated labeled ligands bound to ObR-Fc and IL6Rα-Fc on common beads (solid bars) and in the presence of 10 μM unlabeled IL-6 (hatched bars). (Right) Different receptors on different particles. Cy5.5+ (d) or Cy5+ (e) fluorescence events detected for indicated labeled ligands bound to ObR-Fc and IL6Rα-Fc on separate beads (solid bars) and in the presence of 10 μM unlabeled IL-6 (hatched bars).