Skip to main content
. 1999 Sep 28;96(20):11122–11127. doi: 10.1073/pnas.96.20.11122

Figure 4.

Figure 4

Phosphopeptide–SH2 domain interactions. (a) Displacement of [Cy5]pY-IRS1893–899 from Grb2 by consensus (circles) and nonconsensus (diamonds) phosphopeptides; pY-IRS1893–899 (○); pY-Shc310–324 (●); pY-ObR981–992 (⋄); pY-EpoR450–461(♦); and phenylphosphate (■). [Cy5]pY-IRS1893–899 = 15 nM. (b) Avidity effects. [Cy5]pY-IRS1893–899 (20 nM) displacement from bead-tethered GST-Grb2 with excess pY-IRS1893–899 (60 μM) (solid bars). [Cy5]GST-Grb2 (3 nM; open bars) displacement from bead-tethered pY-IRS1893–899 by excess GST-Grb2 (1 μM).