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cDNA constructs for transient antibody expression in tobacco leaves. (A) CHS-LPH-scFvT84.66-H6-Pw, (B) Ω-LPL-cLightT84.66-Pw, (C) Ω-LPH-cHeavyT84.66-K-Pw expression cassettes. CHS, 5′ UTR of CHS; Ω, Omega leader region of TMV RNA; LPL, plant codon optimized leader peptide derived from murine light chain of TMV-specific mAb24; LPH, plant codon optimized leader peptide derived from murine heavy chain of mAb24; K, KDEL motif; H6, His6 tag; Pw, 3′ UTR of TMV RNA.
