Figure 4.

Characterization of affinity-purified plant-expressed cT84.66. (A) One microgram of murine mAb T84.66 (lane 1) or 10 μl of Protein A purified cT84.66 (lane 2) was analyzed by SDS/PAGE and Coomassie staining. For Western blotting 250 ng of murine mAb T84.66 (lane 1) and 5 μl of cT84.66 (lane 2) was used. Blots were probed with indicated Abs. (B) For ELISA analyses 1.5 ng of mT84.66 and a 1:64 dilution of affinity-purified cT84.66 were applied to microtiter plates coated with 50 ng of CEA/NA3 and detected by the secondary antibodies indicated. Bars represent OD₄₀₅ readings.