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. 1999 Sep 28;96(20):11140–11144. doi: 10.1073/pnas.96.20.11140

Figure 1.

Figure 1

PAGE analysis of UreD-UreF-UreG-apourease complex. (A) Native gel electrophoresis. Samples include UreD-UreF-apourease complex (lane 1), UreD-UreF-UreG-apourease complex (lane 2), and the same sample as shown in lane 2 after activation (lane 3) for 1.5 h at 37°C in the presence of 100 μM NiCl2 and 100 mM bicarbonate in standard activation buffer. (B) SDS/PAGE analysis of UreD-UreF-UreG-apourease complex. Samples include molecular weight markers (phosphorylase b, Mr 97,400; BSA, Mr 66,200; ovalbumin, Mr 45,000; carbonic anhydrase, Mr 31,000; soybean trypsin inhibitor, Mr 21,000; lysozyme, Mr 14,400) (lane 1) and UreD-UreF-UreG-urease apoprotein complex (lane 2).