Table 1.

Michaelis–Menten kinetic data of wild-type and mutant PepA

PepA	Km, mM	kcat, s−1
Wild type (degassed buffer)	0.056  ±  0.003	0.464  ±  0.007
Wild type (5 mM NaHCO3)	0.23  ±  0.01	3.13  ±  0.06
R356A	0.49  ±  0.03	0.227  ±  0.007
R356K	0.29  ±  0.03	0.209  ±  0.008
R356M	0.56  ±  0.04	0.024  ±  0.001
R356E	ND	<3·10−4
K282A	3.9  ±  1.3	0.005  ±  0.001