Polysaccharide density of the capsular layers. (A) After gamma irradiation for 0, 5, 10, 20, 30, or 40 min, we calculated the total amount of GXM (gray bars) contained in each layer, per cell (right axis), and compared this to the layer volume per cell (black line and left axis). See Fig. 1C for the spatial distribution of layers. (B) Using the average amount of GXM per cell (A) and the average volume per layer (A), the average density of total GXM was calculated within the capsule regions. The experiment was duplicated with similar results, and the results of one representative experiment are presented.