TABLE 3.
Scatchard analysis of H99 capsular regions exposed by gamma radiationb
| Irradiation time (min)a | Ka (107, M−1) | No. of binding sites/cell (105) |
|---|---|---|
| None (untreated) | 29.0 | 5.9 |
| 10 | 4.9 | 7.0 |
| 20 | 5 | 7.4 |
| 30 | 12.0 | 4.6 |
| 40 | 8.0 | 7.3 |
a
Time of exposure of C. neoformans strain H99 to 137Cs, which emits gamma radiation at the dose of 1,388 rads/min.
b
Values were determined by Scatchard analysis as described in reference 32. The experiment was done in duplicate, with very similar results being obtained. Data from a representative experiment are shown.