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. 2006 Nov 10;6(1):84–94. doi: 10.1128/EC.00268-06

FIG. 5.

FIG. 5.

SO-GFP localization at septal plugs is Woronin body independent. Strains were grown on Vogel's MM agar plates for 18 h at 30°C. Hyphae were cut with a razor blade and immediately analyzed by light and fluorescence microscopy. (A) Wild-type strain FGSC 2498. Septal pores are plugged in response to the injury (*). Hyphal compartments adjacent to the cut compartment remain intact. (B) HSG8.2.5 (his-3+::Pccg1-so+-gfp+ hex-1). Injured hyphae are not plugged by the Woronin body and lose extensive amounts of cytoplasm. (C to F) Septal pores of HSG8.2.5 hyphae are eventually plugged in a Woronin body-independent manner (black arrow in panel C). SO-GFP localizes to these late-forming plugs (white arrow in panel E). (C) DIC image. (D) Fluorescent image of calcofluor-stained hypha. (E) Fluorescent image with GFP filter. (F) Panels D and E merged. Bars in panels A and B represent 10 μm; the bar in panel C represents 5 μm.