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. 2006 Nov 10;6(1):84–94. doi: 10.1128/EC.00268-06

FIG. 7.

FIG. 7.

SO-GFP localizes to plugs in aging and incompatible hyphae. (A to D) Strain AF-T8 was grown on 2% water agar for 24 h. Hyphae in the inner part of the colony were analyzed by light and fluorescence microscopy. Septa of aging hyphal compartments were plugged (A, arrow). SO-GFP localizes to these plugs (B to D). (A, DIC image; B, GFP; C, calcofluor; D, panels B and C merged). (E to H) Incompatible heterokaryon (strains AF-SG1 and C9-15; Table 1). These two strains contain different het-c haplotypes, resulting in heterokaryon incompatibility. Hyphae show compartmentalization and cell death (dark compartments [*]). Septa are plugged (black arrows), and SO-GFP localizes to these plugs (white arrows). A newly formed hyphal tip has grown into a dead compartment (black triangle in panel G). (E and G) DIC images; hyphae stained with methylene blue. (F and H) GFP filter. All size bars represent 5 μm.