FIG. 5.

Association of capsule expression with supernatant accumulation of GXM-containing vesicles. (A) Capsule expression was higher in yeast cells incubated for 24 h in capsule-inducing media. (B) The profile of GXM detection in ultracentrifugation pellets was very similar to that observed for determinations of capsule size (inset), suggesting a correlation between capsule expression and the production of vesicles containing GXM. GXM concentrations in 100,000 × g fractions under the different conditions of capsule induction were normalized to the number of cells in the culture under each experimental condition.