FIG. 6.

Acapsular cells of C. neoformans bind GXM from extracellular vesicles. Cap67 cells were incubated in the presence of purified vesicles and then analyzed by immunofluorescence with MAb 18B7. Control cells, which were not incubated with MAb 18B7, are shown in the upper panels (a and b). Yeast cells that were incubated in the presence of the vesicular preparation reacted strongly with the antibody to GXM, as shown in the lower panels (c and d). The left panels (a and c) represent cryptococci analyzed under differential interference contrast, while the right panels (b and d) show images in the fluorescence mode. Bar, 2 μm.