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Proposed pathways for incorporation of fatty acids into cryptococcal lipids. The fatty acids can be added exogenously or derived from phospholipids by the PLB activity of PLB1 or phospholipase A1 or A2. The energy-dependent pathway involves the activation of fatty acids by coenzyme A (CoA), whereas the energy-independent pathway involves the transacylase activity of PLB1. The exact mechanism of this transacylase is unknown.
