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. Author manuscript; available in PMC: 2007 Feb 16.
Published in final edited form as: Int J Parasitol. 2006 Mar 13;36(6):723–733. doi: 10.1016/j.ijpara.2006.02.011

Fig. 2.

Fig. 2

Resistin-like molecule (RELM)-β is expressed exclusively in intestinal goblet cells following T. muris infection. Genetically resistant BALB/c mice were infected with 150 T. muris eggs and histologic sections prepared from naïve or infected animals on day 18 p.i. as previously described (Artis et al., 2002, 2004a,b). Paraformaldehyde-fixed tissues were wax-embedded and 5 mm sections stained using a polyclonal rabbit anti-RELMβ antibody (Peprotech, USA) following standard immunofluoresecence protocols. (A) Immunofluorescent staining revealed that RELMβ (staining green) is localised to intestinal goblet cells. (B) RELMβ is expressed exclusively within exocrine vesicles of intestinal goblet cells. Epithelial cell nuclei are stained in blue with DAPI. Blocking peptide confirmed specificity of staining. Bar, 50 μm.