FIGURE 2.

RNA- and DNA-binding ability of wild-type and hybrid proteins. malE–malF (2 fmol) (A) or 1 fmol ompA-3 DNA oligomer (B) were incubated under the conditions described in Materials and Methods. The enzyme concentration used is indicated in the figure. A control, “C,” reaction without enzyme added was performed in all experiments. Binding reactions were applied on a 5% nondenaturing PAA gel. The mobility of free and complexed substrates was detected using the PhosphorImager system from Molecular Dynamics.