FIGURE 4.

Lithium chloride precipitation of the samples abrogates heparin inhibition of the reverse transcriptase. Quantitative RT-PCR amplification of β-actin mRNA from polysome-bound RNA (prepared with heparin), precipitated with either lithium chloride (+LiCl) or ethanol (−LiCl) (A). Aliquots of cytoplasmic Jurkat RNA (prepared in the absence of heparin) were mixed with heparin (650 μg/mL) and subsequently divided in two, one precipitated with lithium chloride (+heparin, +LiCl), or precipitated with ethanol (+heparin, −LiCl). Samples devoid of heparin and precipitated with either LiCl (−heparin, +LiCl) or with ethanol (−heparin, −LiCl) were used as controls. The quantitative amplification of the β-actin mRNA is illustrated for the different conditions (B).