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Determination of tandem U-U wobble conformations in yeast U2 stem I. Imino protons (H3) were assigned by correlation to C4 using 2D H(N)CO (bottom left panel), and correlation of C4 to H5 using 2D HCC (bottom right panel). The 2D HCC H5 assignments were confirmed by 2D 1H–1H TOCSY (upper right panel). The C2 and C4 chemical shifts in the 2D H(N)CO (left panels) identify the hydrogen-bonded carbonyls from their upfield carbon chemical shifts.
