The effect of heterozygote deficiency, gauged by the inbreeding coefficient FIS, on relative bias in estimating allelic dropout rate (ɛ1) averaged over 5000 simulated data sets. Low (ɛ1 = 0.01, solid line) and intermediate (ɛ1 = 0.09, dashed line) allelic dropout rates were estimated at three false allele error rates (•, ɛ2 = 0.0015; ○, ɛ2 = 0.04; ▾, ɛ2 = 0.08). Other parameter values were He = 0.85 and n = 100.