FIG. 7.

Analysis of MK2/MK3 DKO mice. (A) Western blot detection of p38 MAPK levels in total lysates from MK2/MK3 DKO and, as controls, in MK2- and MK3-single knockout and WT mouse tissues. (B) Peritoneal-derived macrophages were stimulated with 1 μg/ml LPS for the times indicated. The TTP level in total lysates from WT, MK2^−/−, MK3^−/−, and MK2/MK3 DKO cells was analyzed by Western blotting. (C) TNF ELISA of macrophage culture supernatants. BMDMs were cultivated for 7 days, harvested, and counted, and equal numbers of cells were transferred to a 96-well plate. Cells were induced with 1 μg/ml LPS or left untreated as control for 2 and 6 h. TNF levels in the supernatants were measured by ELISA. For each genotype, three independent measurements were made; average and standard deviations are shown. The experiment is representative of two similar experiments.