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. 2006 Oct 16;27(1):135–146. doi: 10.1128/MCB.01283-06

FIG. 1.

FIG. 1.

Loss of dCBP function causes defects in chromosomal condensation and integrity. Chromosome spreads of Kc cells treated with water (A) or dsRNAs for dCBP (B and C). The chromosomes were visualized by staining with propidium iodide. Control chromosomes are evenly and tightly condensed while the chromosomes from the dCBP dsRNA-treated cells are only partially condensed (B) or shredded (C). Mitotic chromosomes from heat-shocked hsgal4/+ (D to F) and hp12.3; hsgal4/+ (G to J) third instar larvae are shown. The heat-shocked hsgal4/+ larval brain cells have normally condensed mitotic chromosomes (D to F), demonstrating that the heat shock treatment does not affect the morphology of the chromosomes. The heat-shocked hp12.3 larval brains are identical to the heat-shocked hsgal4/+ (not shown). The hp12.3; hsgal4/+ larval brain cells contained chromosomes that failed to condense properly and appeared shredded (G to J). (K) A Western analysis of the gal4/+ and hp12.3; hsgal4/+ larval brains. dCBP is significantly reduced in the heat-shocked hp12.3; hsgal4/+ brains. Heat shock does not affect the expression of dCBP in the hsgal4/+ controls. Gsα is an internal loading and transfer control.