FIG. 3.

Loss of dCBP function does not affect G₂ timing, and HU suppresses BrdU incorporation. (A) Discs from the heat-shocked hp12.3; hsgal4/+ and hsgal4/+ larvae were incubated in BrdU for 1, 2, 3.5, 4.5, and 6 h and were then assayed for mitotic cells that had incorporated the BrdU. There is no detectable, significant difference in the lengths of G₂ seen in the two populations. (B) Quantification of the HU-treated discs pooled from three experiments. A Student's t test determined that there are no differences between the means of the control and experimental discs. (C) Discs from the heat-shocked hp12.3; hsgal4/+ and hsgal4/+ larvae were treated with HU for 2.5 h and then treated with BrdU for 30 min. HU treatment suppresses BrdU incorporation in control and dCBP siRNA-expressing cells. (D) Discs from the heat-shocked hp12.3; hsgal4/+ and hsgal4/+ larvae were incubated in BrdU for 30 min, and then half of the control and experimental discs were treated with HU for 4.5 to 5 h. Significantly more mitotic cells are BrdU positive in the HU-treated hp12.3; hsgal4/+ discs than in the HU-treated hsgal4/+ discs. The data represent three pooled experiments. A Student's t test shows that the mean between the two populations is significantly different. The asterisk indicates a significant difference (P < 0.01).