FIG. 6.

Antagonistic effect of the UGCAUG silencer elements and intronic enhancer element on exon 4 definition. (A) Diagram illustrating the wild-type and mutant reporter minigene constructs used in this experiments. An “X” indicates mutations of either UGCAUG to UGACUG at −34 and+45 position, CAGGUAAGAC to CAGCAUAGAC at the pseudo 5′ splice site (5′ss), or UUUUUAUUUUC to GUGUGAUGGUC at U-tract, respectively. (B) RT-PCR assay of total RNA isolated from HeLa or CA77 Cells transfected with the calcitonin/CGRP reporter minigenes. (C) Quantification of the RT-PCR analysis shown in panel B.