TABLE 1.
Summary of yeast two-hybrid interactions between NirA, KapK, and NplAa
| Vector | Interactionb
|
|||
|---|---|---|---|---|
| AD-Tag | AD-NplA | AD-KapK | AD-NirA | |
| BD-p53 | + | − | − | − |
| BD-NirA | − | + | + | + |
| BD-KapK | − | + | ND | + |
a
Yeast two-hybrid interactions were monitored by complementation of the ade2 mutation. Bait vectors containing the GAL4 DNA-binding domain (BD) were constructed to result in a fusion with a control protein (BD-p53), with NirA1-229 (BD-NirA), or with KapK (BD-KapK). Prey vectors containing the GAL4 AD were constructed to result in a fusion with a control protein (T-antigen, AD-Tag, interacting with p53), with NplA (AD-NplA), with KapK (AD-KapK), or with NirA (AD-NirA). The BD-NirA-AD-NirA interaction serves as an additional positive control due to the homodimerization element in the NirA protein (55).
b
+, Interaction; −, no interaction; ND, not determined.