FIG. 4.

Gel filtration analysis of retromer subunits. Triton X-100 extracts (A) and cytosol (B) from HeLa cells, prepared as indicated in Materials and Methods, were subjected to gel filtration on a calibrated Superdex 200 HR column. Samples representing 2% of the volume of each fraction were analyzed by 4 to 20% acrylamide gradient SDS-PAGE and immunoblotting with antibodies to each of the retromer subunits. The void volume (V₀) and the positions, molecular masses, and Stokes radii of standard proteins are indicated at the top.