FIG. 8.

GAP-defective mutant Bcr and Abr localize to phagocytic cups during phagocytosis of opsonized zymosan particles. After transfection with the indicated plasmids, RAW cells were serum starved and then incubated with Texas Red-coupled zymosan particles at a ratio of 1:25. EGFP-expressing cells appear green, zymosan is red, and nuclei are blue. Images with different fluorescences were merged, as indicated above the panels, to identify EGFP signal localizing to phagosomes (arrowheads point to examples). Bars, 20 μm. Note that the images of the EGFP-Abr R683A-transfected cells are shown at a lower magnification than the others, in order to capture one elongated cell.