FIG. 3.

Kar4-dependent clusters after pheromone treatment. Strains MS3213 (wild type) and MS3215 (kar4Δ) were exposed to pheromone (+) or mock treated with methanol (−) for 90 min. RNA was extracted and prepared for microarray analysis as described in Materials and Methods. Genes were clustered according to their normalized expression profiles. Specifically, we identified seven clusters that show Kar4-independent pheromone induction (cluster 1), Kar4-independent repression in pheromone (cluster 2), Kar4-dependent pheromone induction (cluster 3), Kar4-dependent repression in pheromone (cluster 4), constitutive expression in wild-type cells but repression by pheromone in the kar4Δ strain (cluster 5), constitutive expression in wild-type cells but induction by pheromone in the kar4Δ strain (cluster 6), and constitutive expression in wild-type cells but higher levels of mitotic expression in the kar4Δ strain (cluster 7).