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. 2006 Nov 13;27(3):1096–1111. doi: 10.1128/MCB.01750-06

FIG. 3.

FIG. 3.

Annexin V induction in CD8+ T-cell apoptosis is linked to loss of core 1 O-glycan sialylation by a posttranscriptional mechanism. (A) CD8+ T cells from ST3Gal-ITg and littermate wild-type mice were activated in vitro with the indicated stimuli and analyzed for PNA ligand levels. Flow cytometry plots depict live (7-AAD−) CD8+ T cells; numbers indicate percentages of cells in the upper right quadrant and are representative of results from three separate experiments. PNA ligand levels were assessed by flow cytometry among stimulated cells (at 72 h) and compared to unstimulated CD8+ T cells to calculate relative induction. aCD3, anti-CD3. (B) Percentage of live (7-AAD−) annexin V+ CD8+ T cells at the indicated time points after activation with the indicated stimuli. (C) Reverse transcription-PCR analysis of ST3Gal-I transgene expression (normalized to 18s rRNA expression) was performed on purified CD8+ T cells isolated at 0, 24, 48, and 72 h post-anti-CD3 activation. The intensity ratio represents the ratio of ST3Gal-I transgene to 18S bands as assessed by densitometry.