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. 2006 Nov 13;27(3):1096–1111. doi: 10.1128/MCB.01750-06

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Copyright © 2007, American Society for Microbiology

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FIG. 7. — Loss of ST3Gal-I overcomes Bcl-2 expression to induce CD8⁺ T-cell apoptosis in vivo, but not in vitro, coincident with the expression of unsialylated core 1 O-glycans. (A) Flow cytometric analysis of live (7-AAD⁻) CD8⁺ cells from the lymph nodes and spleens of wild-type, Bcl-2^Tg, ST3Gal-I^Δ/Δ, and Bcl-2^Tg/ST3Gal-I^Δ/Δ mice. Percentages of annexin V⁺ cells are shown in the graph at the right depicting means ± standard errors of the means (n = 4). Total numbers of CD8 (B) and CD4 (C) SP cells from the lymphoid tissues of wild-type, Bcl-2^Tg, ST3Gal-I^Δ/Δ, and Bcl-2^Tg/ST3Gal-I^Δ/Δ mice. (D) Total numbers of Vβ8⁺ CD8⁺ T cells from lymph nodes and spleens of indicated mice at 0, 2, and 10 days postinjection with SEB. (E) PNA ligands and annexin V reactivity were assessed by flow cytometry among live (7-AAD⁻) Vβ8⁺ CD8⁺ T cells removed from Bcl-2^Tg and Bcl-2^Tg/ST3Gal-I^Δ/Δ mice 10 days post-SEB injection. (F) Percentages of live (7-AAD⁻) Vβ8⁺ CD8⁺ T cells during the 48 h in culture following isolation at 2 days post-SEB injection. In all cases, cells were enumerated by hemacytometer counts in combination with percentages obtained by flow cytometric analysis. Data are presented as means ± standard errors of the means (n = 6 [A to C] and n = 3 [D, F]). , statistically significant difference from wild-type littermates (P < 0.05, unpaired t test).

Inline graphic — Loss of ST3Gal-I overcomes Bcl-2 expression to induce CD8⁺ T-cell apoptosis in vivo, but not in vitro, coincident with the expression of unsialylated core 1 O-glycans. (A) Flow cytometric analysis of live (7-AAD⁻) CD8⁺ cells from the lymph nodes and spleens of wild-type, Bcl-2^Tg, ST3Gal-I^Δ/Δ, and Bcl-2^Tg/ST3Gal-I^Δ/Δ mice. Percentages of annexin V⁺ cells are shown in the graph at the right depicting means ± standard errors of the means (n = 4). Total numbers of CD8 (B) and CD4 (C) SP cells from the lymphoid tissues of wild-type, Bcl-2^Tg, ST3Gal-I^Δ/Δ, and Bcl-2^Tg/ST3Gal-I^Δ/Δ mice. (D) Total numbers of Vβ8⁺ CD8⁺ T cells from lymph nodes and spleens of indicated mice at 0, 2, and 10 days postinjection with SEB. (E) PNA ligands and annexin V reactivity were assessed by flow cytometry among live (7-AAD⁻) Vβ8⁺ CD8⁺ T cells removed from Bcl-2^Tg and Bcl-2^Tg/ST3Gal-I^Δ/Δ mice 10 days post-SEB injection. (F) Percentages of live (7-AAD⁻) Vβ8⁺ CD8⁺ T cells during the 48 h in culture following isolation at 2 days post-SEB injection. In all cases, cells were enumerated by hemacytometer counts in combination with percentages obtained by flow cytometric analysis. Data are presented as means ± standard errors of the means (n = 6 [A to C] and n = 3 [D, F]). , statistically significant difference from wild-type littermates (P < 0.05, unpaired t test).